Streptozotocin (STZ) is toxic to pancreatic islet insulin-producing b-cells, thus inducing diabetes. Other models of diabetes include genetic models and the use of a prolonged high-fat diet regimen. The STZ model provides a good, simple screening tool to test compounds and to further understand the complex development of neuropathic pain.
Animals will not be supplemented with insulin, except possibly as a control, since it may hinder the development of STZ-induced neuropathy4,5. Also, rats should be housed no more than 2 per cage and should be changed at least 3 times per week (if in standard housing), as they tend to drink a lot and soil their cage quickly.