Diets with tamoxifen activate CreER
Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor. Inotiv Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of doxycycline-containing diets for tet regulated systems. If you have any questions, don't hesitate to consult a nutritionist.
Teklad stocks tamoxifen and tamoxifen citrate
Tamoxifen is most often added to the diet as a tamoxifen-sucrose mixture. The options below are made frequently and often available for quicker shipment.
Tamoxifen containing diets can be further customized with use of different base diets, adjustment of sucrose (or other palatant) levels, tamoxifen dose, or food color.
1 Assumes 20-25 g body weight and three-four g intake
2 Tamoxifen citrate is 66% tamoxifen
3 Referred to as TAM400/CreER in Europe (TD.55125 )
Please add 10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.
These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.
Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.
If intake seems to be a problem:
1 Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010. Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts. Transgenic Res 19:715-725.
2 Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010. Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. Proc Natl Acad Sci USA 107:16320-16324.
3 Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011. Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice. PLoS One 6:e21055.
4 Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007. Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate. Genesis 45:11-16.
5 Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009. Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12-15.
6 Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. PCirc Res 106:559-572.
7 Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011. Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression. PLoS One 6:e22589.
8 Welle S, Burgess K, Thornton CA, Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.
If you have any questions regarding tamoxifen diets, please don’t hesitate to contact a nutritionist at firstname.lastname@example.org.