Antibody induced arthritis via monoclonal antibodies (mAb) to collagen have been shown to induce arthritis lesions in various strains of mice, with combinations of three or more antibodies resulting in severe arthritis with marked destruction of articular cartilage.
On study day 0, mice are injected intravenously (IV) with 3 mg of monoclonal antibody (mAb). On day 3, mice are dosed intraperitoneally (IP) with E. coli lipopolysaccharide (LPS) (25 µg/mouse).
Arthritis has been demonstrated to occur within 48 to 72 hours post-injection with antibodies, resulting in disease that persists for at least three weeks1, 2. E. coli lipopolysaccharide (LPS), a strong inducer of pro-inflammatory cytokines, acts synergistically in the induction of antibody-mediated arthritis, and negates the multiple epitope specificity of autoantibodies in the model3.
Treatment is initiated approximately 1 to 2 hours prior to mAb injection (d0) and continues for 14 days (days 0 through 13).
Clinical scores are given for each of the paws (right front, left front, right rear, left rear) on study days 0 through 14.
Fore paws, hind paws/ankles, and knees are given scores of 0 to 5 for inflammation, pannus formation, cartilage damage, bone resorption, and periosteal new bone formation according to these methods.
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