Typically heterozygous rats are cryo-recovered in 10-12 weeks to an age of 7 weeks old. Subsequently homozygous rats to an age of 7 weeks old can be produced within 20-22 weeks.
- Homozygotes exhibit multiple phenotypes involving intestinal, respiratory, and reproductive phenotypes (see Description).
Availability: Cryopreserved as a mix of Heterozygous & WT embryos
CFTR knockout rats possess a 16 bp deletion in exon 3 of the Cystic Fibrosis transmembrane conductance regulator (CFTR), resulting in loss of protein expression.
CFTR knockout rats lack CFTR protein as demonstrated by western blot. CFTR KO rats develop intestinal obstructions shortly after weaning leading to weight loss and mortality. CFTR KO rats also exhibit decreased trachea circumference as well as submucosal gland volume. Stored nasal mucus volume is increased. Abnormal dentition is observed, and the vas deferens is absent in male CFTR knockout rats.
gem~gem rat~gem rats~knockout rats~knockout rat~CFTR knockout rats~CFTR~CFTR rat~CFTR knockout~HsdSage:SD-Cftrtm1Sage~Cryopreserved~Cystic Fibrosis transmembrane conductance regulator~Cystic Fibrosis~CF Transmembrane Conductance Regulator~Cystic Fibrosis Transmembrane Conductance Regulator~Channel Conductance-Controlling ATPase~CAMP-Dependent Chloride Channel~DJ760C5.1~CFTR/MRP~TNR-CFTR~ABC35~ABCC7~MRP7~Cystic Fibrosis Transmembrane Conductance Regulator, ATP-Binding Cassette (Sub-Family C, Member 7)~Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7)~Cystic Fibrosis Transmembrane Conductance Regulating~ATP-Binding Cassette Sub-Family C, Member~ATP-Binding Cassette Sub-Family C Member~EC 126.96.36.199~EC 188.8.131.52~EC 3.6.3~CF
Origin: The CFTR KO rat model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo, then Envigo was acquired by Inotiv in 2021.