Typically heterozygous rats are cryo-recovered in 10-12 weeks to an age of 7 weeks old. Subsequently homozygous rats to an age of 7 weeks old can be produced within 20-22 weeks.
Availability: Cryopreserved as a mix of Heterozygous & WT embryos
Zygosity: Heterozygous as live colony
CFTR knockout rats possess a 16 bp deletion in exon 3 of the Cystic Fibrosis transmembrane conductance regulator (CFTR), resulting in loss of protein expression.
CFTR knockout rats lack CFTR protein as demonstrated by western blot. CFTR KO rats develop intestinal obstructions shortly after weaning leading to weight loss and mortality. CFTR KO rats also exhibit decreased trachea circumference as well as submucosal gland volume. Stored nasal mucus volume is increased. Abnormal dentition is observed, and the vas deferens is absent in male CFTR knockout rats.