Cryopreserved as Heterozygous Embryos
Typically heterozygous rats are cryo-recovered in 10-12 weeks to an age of 7 weeks old. Subsequently homozygous rats to an age of 7 weeks old can be produced within 20-22 weeks.
- Background strain: Long Evans Hooded
- Cre recombinase driven by endogenous 5Ht3a promoter
- No observed ectopic expression of cre
- Targeted insertion eliminates possible gene disruption that may occur in random insertion technologies such as BAC
Availability: Cryopreserved as homozygous embryos
Zygosity genotype: Homozygous
This model expresses cre-recombinase under the control of the endogenous 5’- hydroxytryptamine receptor 3A (5Ht3a) promoter enabling specific expression in 5Ht3a positive serotonergic neurons. This model possesses a targeted insertion of (T2A)-cre immediately before the translational stop in the open reading frame of the 5Ht3a gene. The 5Ht3a-Cre rat is useful for applications requiring tissue specific expression, including optogenetics and breeding with transgenic floxed lines.
gem~gem rat~gem rats~knockin rats~knockin rat~5Ht3a-Cre knockin rats~5Ht3a-Cre~HsdSage:LE-5HT3aem1(T2A-Cre)Sage~5Ht3a~cre~Serotonin~Hydroxytryptamine~5-Hydroxytryptamine Receptor 3A~5-HT3A~5-HT3R~5-Hydroxytryptamine (Serotonin) Receptor 3A, Ionotropic~Serotonin-Gated Ion Channel Receptor~5-Hydroxytryptamine Receptor 3~Serotonin Receptor 3A~5-HT-3~5HT3R~HTR3~5-Hydroxytryptamine (Serotonin) Receptor 3A~5HT3 Serotonin Receptor~5-HT3-A~HTR3A
Origin: The 5Ht3a-Cre KI rat model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo, then Envigo was acquired by Inotiv in 2021.