On study day 0, mice are given intraperitoneal injections of zymosan (1 mg) in sterile saline to induce peritonitis.
Inflammation is the response of vascularized tissues to injury, irritation, and infection. Acute inflammation is most often associated with a neutrophil-rich cellular infiltrate and is generally resolved in a period of days, whereas chronic inflammation is characterized by a cellular infiltrate containing many more mononuclear cells (monocyte/macrophages and lymphocytes).1,2,3 The ability to investigate the role of individual inflammatory mediators in acute inflammation, such as complement proteins, prostaglandins, leukotrienes, cytokines, and chemokines, has been aided by the availability of simple models of sterile peritonitis, which allow the accurate quantitation of leukocyte recruitment into an easily accessible serosal cavity.4-10 Recently, sterile peritonitis models have proved useful for the analysis of animals in which specific inflammatory mediators or their receptors have been ablated genetically or targeted by neutralizing antibodies.11,12
The peritoneal cavity is lavaged, the lavage fluid is transferred to a 15 ml tube and the peritoneal exudate cells are pelleted by centrifugation. The pellets are re-suspended and single cell suspensions are prepared by thoroughly washing each pellet. The cell suspensions are spun down in a Cytospin® collection funnel with an attached slide. The slides are dried overnight followed by staining with a Diff-Quick stain. The cell differentiation counts (granulocyte, monocyte/macrophage, lymphocyte, etc.) are quantified under a light microscope. Cell counts are performed by counting squares on a Bright-Line Hemocytometer.
For examples of positive controls, please contact us.
Experimental peritonitis models have proved to be an indispensable tool for the explanation of acute inflammatory processes, the mechanisms of action of anti-inflammatory drugs, and are increasingly being used to test novel anti-inflammatory therapies, including anti-chemokine and anti-chemokine receptor strategies.13,14
For more information about Zymosan Induced Peritonitis contact us here.